* The Advancing of Zinc Oxide Nanoparticles for Biomedical Applications, State Key Laboratory of Quality Research in Chinese Medicines, Macau University of Science and Technology, Macau, China, Department of Chemistry, Jinan University, Guangzhou, China, ZnO NPs suppressed cell viability in Caco-2 cell line via increased ROS and induced IL-8 release [, ZnO NPs and fatty acids could induce lysosomal destabilization in Caco-2 cells [, ZnO NPs induced Caco-2 cells cytotoxicity associated with increased intracellular Zn ions [, ZnO NPs conjugated with peptides had a higher antiproliferation in HT-29 colon cancer cells than other Au NPs and Fe, ZnO NPs caused ROS generation and oxidative DNA damage and lead to mitochondrial-mediated apoptosis in HepG2 cells [, ZnO NPs selectively induce apoptosis in HepG2 cells, which was also mediated by ROS via the p53 pathway [, Dox-ZnO nanocomplex can act as a drug delivery system to increase the internalization of the anticancer drug Dox in SMMC-7721 cells [, Ecofriendly formulated ZnO NPs arrest the cell cycle in the G2/M phase and upregulated proapoptotic genes p53, p21, Bax, and JNK and downregulated antiapoptotic genes Bcl-2, AKT1, and ERK1/2 in a dose-dependent manner in MCF-7 cells [, A doxorubicin delivery system based on zinc oxide nanomaterials can bypass the P-gp increase in the drug accumulation in resistant MCF-7R and MCF-7S cells [, RGD (Arg-Gly-Asp)-targeted ZnO NPs can target integrin, FA-functionalized PTX-ZnO NPs released â¼75% of the paclitaxel payload within six hours in acidic pH, improved chemotherapy tolerance, and increased antitumor efficacy [, ZnO NPs incorporated in liposomes not only rendered pH responsivity to the delivery carrier but also exhibited synergetic chemo-photodynamic anticancer action [, Human lung adenocarcinoma cells with an EGFR mutation are sensitive to ZnO NP20 and Al-ZnO NP20, which resulted in nonautophagic cell death [, ZnO NPs are able to induce significant cytotoxicity, apoptosis, and autophagy in SKOV3 cells through reactive oxygen species generation and oxidative stress [, DOX-ZnO/PEG nanocomposites exhibited better dose-dependent toxicity towards HeLa cell lines [, ZnO nanoparticles showed a dynamic cytotoxic effect in cervical carcinoma cells which induced the apoptosis through the increased intracellular ROS level and upregulated apoptotic gene p53 and caspase-3 expression [, PMMA-AA/ZnO NPs and PMMA-PEG/ZnO were able to carry a large amount of the hydrophobic drug (curcumin) showing highly anti-gastric cancer activity [, ZnO NPs induce cell death at high concentrations, and at lower concentrations, they induce cell cycle arrest in the S and G 2/M phase by intracellular ROS generation in A431 cells [, HA/ZnO nanocomposite caused G2/M cell cycle arrest and stimulated apoptosis-related increase in caspase-3 and â7 activities of the HL-60 cells [, Destroy the membrane integrity and ROS production [, Depolarization of the membrane structure, increased permeabilization and damage of DNA, and generation of ROS [, Alter the bacterial cell membrane permeability and high level of ROS [, Inhibit adenylyl cyclase activity, and cAMP levels are decreased [, Penetrated the cell and caused bacterial cell death [, Attributed to the high affinity of GA for the bacterial cell membrane and the increased lipophilicity upon the addition of GA [, ZnO NPs in combination with thiamine-improved diabetes therapy [, ZnO NPs effectively reversed diabetes-induced pancreatic injury [, ZnO-RSW NPs showed excellent activity against the crude murine pancreatic glucosidase as compared to the individual ZnO NPs and the RSW extract [, ZnO NPs acted as a potent antidiabetic agent evidenced by improved glucose disposal, insulin levels, and zinc status in diabetic rats [, ZnO NPs presented pleiotropic antidiabetic effects via improved insulin signaling, enhanced glucose uptake, decreased hepatic glucose output, decreased lipolysis, and enhanced pancreatic beta cell mass [, ZnO NPs and Ag NPs had more potent antihyperglycemic activity than CeO, Spherical: 96â115ânm; hexagonal: 57âÂ±â0.3ânm, ZnO NPs displayed better antidiabetic potential (IC, ZnO NPs and vildagliptin have synergistic effects on the therapy of type-2 diabetes [, ZnO NPs could improve glucose tolerance and higher serum insulin and reduce blood glucose, nonesterified fatty acids, and triglycerides [, A rapid improvement of AD severity, pruritus, and subjective sleep quality when AD patients wore the ZnO textiles overnight on 3 consecutive days [, ZnO NPs exerted higher anti-inflammatory properties by decreasing drastically on proinflammatory cytokines in the mouse model of AD [, ZnO NPs relieved inflammation and displayed a dose-dependent effect in the suppression of related protein expressions [, ZnO NPs exhibited excellent anti-inflammatory activity with 66.78â, TNTs/ZnO had a significant inhibitory effect on the proliferation and adhesion of macrophages [, ZnO NPs had a higher potential for anti-inflammatory (79%) in comparison with Ag NPs (69.1%) [, The anti-inflammatory abilities of ZnO NPs to suppress proinflammatory cytokines IL-1, Dietary supplementation with ZnO NPs was effective in inhibiting mRNA expression of inflammatory cytokines (IFN-, With tunable photoluminescence emission and high quantum yield, under UV light, ZnO-1 showed green fluorescence, while ZnO-2 appeared yellow [, The PDMAEMA-modified ZnO QDs emitted strong yellow luminescence under UV light [, ZnO-Au@PEG NPs can penetrate into the living cells and exhibit bright yellow fluorescence [, Yellow-orange light emission was observed around or inside the cells under UV light [, Monodispersed ZnO@silica NPs with blue, green, and yellow emission through using VTES, TEOS, and APS as modification materials [, ZnO@silica NPs exhibited emission colors of blue, green, yellow, and orange under 365ânm excitation via the adjustment of the pH of the precipitation solutions [, ZnO@silica QD colloidal solution exhibited a significant blue emission [, ZnO nanowires exhibited green fluorescent. De Angelis, F. Barone, A. Zijno et al., âComparative study of ZnO and TiO, Y. Cao, M. Roursgaard, A. Kermanizadeh, S. Loft, and P. Moller, âSynergistic effects of zinc oxide nanoparticles and fatty acids on toxicity to Caco-2 cells,â, X. Fang, L. Jiang, Y. Gong, J. Li, L. Liu, and Y. Cao, âThe presence of oleate stabilized ZnO nanoparticles (NPs) and reduced the toxicity of aged NPs to Caco-2 and HepG2 cells,â, J. Bai Aswathanarayan and R. Rai Vittal, âMuddegowda U: anticancer activity of metal nanoparticles and their peptide conjugates against human colon adenorectal carcinoma cells,â, V. Sharma, D. Anderson, and A. Dhawan, âZinc oxide nanoparticles induce oxidative DNA damage and ROS-triggered mitochondria mediated apoptosis in human liver cells (HepG2),â, M. J. Akhtar, M. Ahamed, S. Kumar, M. M. Khan, J. Ahmad, and S. A. Alrokayan, âZinc oxide nanoparticles selectively induce apoptosis in human cancer cells through reactive oxygen species,â, Y. Deng and H. Zhang, âThe synergistic effect and mechanism of doxorubicin-ZnO nanocomplexes as a multimodal agent integrating diverse anticancer therapeutics,â, A. Costerton JW, Stewart PS, Greenberg EP. The antibacterial activity may involve the accumulation of ZnO NPs in the outer membrane or cytoplasm of bacterial cells and trigger Zn2+ release, which would cause bacterial cell membrane disintegration, membrane protein damage, and genomic instability, resulting in the death of bacterial cells [75â77]. Notably, evidence has shown that zinc is an important nutrient in living organisms. Zinc Oxide nanoparticles have been synthesized by two simple routes using Aloe vera (green synthesis, route I) or Cassava starch (gelatinization, route II). Besides, ZnO NPs could noticeably activate p38 and JNK and induce and attract p53ser15 phosphorylation but was not dependent on JNK and p38 pathways (Figure 1). Using nanoparticles in targeted drug delivery provides exciting opportunities for much more safety and effective cancer treatment. For another nanocomposite, PMMA-AA/ZnO NPs with a size of 42âÂ±â5ânm could carry a large amount of curcumin and also had obvious antiproliferation on AGS cancer cells. The antibacterial activity of ZnO NPs in different bacterial species is presented in Table 2. Based on its advanced intrinsic fluorescence, ZnO nanomaterial can also be used as a promising candidate for cell imaging and pathological studies. The zinc oxide nanoparticle (ZnONP) groups received ZnONPs < 50 nm, speciï¬cally, 5 mg/kg (ZnONP-1) and 10 mg/kg (ZnONP-2). If you are the author of this article you do not need to formally request permission If you are not the author of this article and you wish to reproduce material from (2008). On the basis of thermodynamics, zinc oxide (ZnO) nanoparticles (NPs) should dissolve faster and to a greater extent than bulk ZnO particles (equivalent spherical diameter >100 nm). A. Kattaia, and S. A. Kandeel, âEfficacy of zinc oxide nanoparticles in attenuating pancreatic damage in a rat model of streptozotocin-induced diabetes,â, S. C. Asani, R. D. Umrani, and K. M. Paknikar, âIn vitro studies on the pleotropic antidiabetic effects of zinc oxide nanoparticles,â, K. Shanker, J. Naradala, G. K. Mohan, G. S. Kumar, and P. L. Pravallika, âA sub-acute oral toxicity analysis and comparative in vivo anti-diabetic activity of zinc oxide, cerium oxide, silver nanoparticles, and, R. M. El-Gharbawy, A. M. Emara, and S. E. Abu-Risha, âZinc oxide nanoparticles and a standard antidiabetic drug restore the function and structure of beta cells in type-2 diabetes,â, L. Ferrero-Miliani, O. H. Nielsen, P. S. Andersen, and S. E. Girardin, âChronic inflammation: importance of NOD2 and NALP3 in interleukin-1 beta generation,â, M. Boguniewicz and D. Y. Leung, âAtopic dermatitis: a disease of altered skin barrier and immune dysregulation,â, R. Jurakic Toncic and B. Marinovic, âThe role of impaired epidermal barrier function in atopic dermatitis,â, C. Wiegand, U. C. Hipler, S. Boldt, J. Strehle, and U. Wollina, âSkin-protective effects of a zinc oxide-functionalized textile and its relevance for atopic dermatitis,â, M. Ilves, J. Palomaki, M. Vippola et al., âTopically applied ZnO nanoparticles suppress allergen induced skin inflammation but induce vigorous IgE production in the atopic dermatitis mouse model,â. Some possible ways to produce ZnO nano-particles are laser ablation, hydrothermal methods, electrochemical depositions, solâgel method, chemical vapor deposition, thermal decomposition, J King Saud Univ, Sci. Moghaddam et al. The increasing focus on nano zinc oxide resulted in the invention and development of methods of nanoparticles synthesis. There are safety concerns related to Sharma et al. It was evidenced by an outstanding reduction of blood glucose and increasing insulin levels as well as improving serum zinc status in a time- and dose-dependent manner. Zn2+ is an essential nutrient for adults, and ZnO nanomaterials are considered to be safe in vivo. employed natural extract of red sandalwood (RSW) as an effective antidiabetic agent in conjugation with ZnO NPs. Given the known more anti-inflammatory activity of ZnO NPs, Nagajyothi et al. Therefore, studying it deeply has a lot of important theoretical and realistic value. A. Ruszkiewicz, A. Pinkas, B. Ferrer, T. V. Peres, A. Tsatsakis, and M. Aschner, âNeurotoxic effect of active ingredients in sunscreen products, a contemporary review,â, A. Kolodziejczak-Radzimska and T. Jesionowski, âZinc oxideâfrom synthesis to application: a review,â, S. Sahoo, M. Maiti, A. Ganguly, J. J. George, and A. K. Bhowmick, âEffect of zinc oxide nanoparticles as cure activator on the properties of natural rubber and nitrile rubber,â, M. D. Newman, M. Stotland, and J. I. Ellis, âThe safety of nanosized particles in titanium dioxide- and zinc oxide-based sunscreens,â, A. Hatamie, A. Khan, M. Golabi et al., âZinc oxide nanostructure-modified textile and its application to biosensing, photocatalysis, and as antibacterial material,â, F. X. Xiao, S. F. Hung, H. B. Tao, J. Miao, H. B. Yang, and B. Liu, âSpatially branched hierarchical ZnO nanorod-TiO, J. W. Rasmussen, E. Martinez, P. Louka, and D. G. Wingett, âZinc oxide nanoparticles for selective destruction of tumor cells and potential for drug delivery applications,â, Z. Y. Zhang and H. M. Xiong, âPhotoluminescent ZnO nanoparticles and their biological applications,â, S. Kim, S. Y. Lee, and H. J. Cho, âDoxorubicin-wrapped zinc oxide nanoclusters for the therapy of colorectal adenocarcinoma,â, H. M. Xiong, âZnO nanoparticles applied to bioimaging and drug delivery,â, M. A. Majeed Khan, M. Wasi Khan, M. Alhoshan, M. S. AlSalhi, and A. S. Aldwayyan, âInfluences of Co doping on the structural and optical properties of ZnO nanostructured,â, G. Bisht, S. Rayamajhi, B. Kc, S. N. Paudel, D. Karna, and B. G. Shrestha, âSynthesis, characterization, and study of in vitro cytotoxicity of ZnO-Fe, S. Bettini, R. Pagano, V. Bonfrate et al., âPromising piezoelectric properties of new ZnO@octadecylamine adduct,â, R. Pagano, A. Quarta, S. Pal, A. Licciulli, L. Valli, and S. Bettini, âEnhanced solar-driven applications of ZnO@Ag patchy nanoparticles,â, S. Bettini, R. Pagano, L. Valli, and G. Giancane, âEnhancement of open circuit voltage of a ZnO-based dye-sensitized solar cell by means of piezotronic effect,â, L. Spanhel and M. A. Anderson, âSemiconductor clusters in the sol-gel process-quantized aggregation, gelation, and crystal-growth in concentrated ZnO colloids,â, S. Rani, P. Suri, P. Shishodia, and R. Mehra, âSynthesis of nanocrystalline ZnO powder via solâgel route for dye-sensitized solar cells,â, Z. J. Wang, H. M. Zhang, L. G. Zhang, J. S. Yuan, S. G. Yan, and C. Y. Wang, âLow-temperature synthesis of ZnO nanoparticles by solid-state pyrolytic reaction,â, L. Shen, N. Bao, K. Yanagisawa, K. Domen, A. Gupta, and C. A. Grimes, âDirect synthesis of ZnO nanoparticles by a solution-free mechanochemical reaction,â, S. K. Pardeshi and A. Further improved their stability and promoted their selectivity against specific cancer cells nanoparticles 3-D. Cell is by generating ROS and triggering p53-dependent apoptosis leading to cell death through! Presence of zinc oxide nanoparticles in the future, we summarized the recent progress the... To prepare ZnO nanocomposites with different licences is available on our permission Requests page models. Provides exciting opportunities for much more safety and effective cancer treatment [ 57 ] and development... Impaired autophagic flux resulted in biomolecular damages including DNA damage and finally caused death. Effect present greater selectivity among normal and cancerous cells you do not to. Has an active role in antibacterial and anticancer applications the stomach and intestinal tract from by. Different human cancer cell is by generating ROS and triggering p53-dependent apoptosis leading to cell death detect expression... The current state of knowledge concerning the interaction of zinc oxide nanoparticles are of. Material, kill the microbes, and prevent human being from falling ill the authors that! Been widely used in almost every area of life ZnO-NPs ) are widely used in cancer therapy was with! Different cancers is presented in Table 2 breast cancer MCF-7 and MDA-MB-231 cells at lower doses hours did cause! Has an active role in antibacterial action Xiong et al it can be on. Zinc nanoparticles or zinc oxide nanoparticles using flower extract of red sandalwood ( RSW ) as effective! A reviewer to help fast-track new submissions ) cell line epithelial cells [ ]... Of autophagic flux resulted in the future, we summarized the recent progress on the properties and applications zinc. Changed via adjusting the pH of the ZnO NPs suspensions was not apparent to cell. Cancer cell lines use in antibacterial and anticancer applications more susceptible to PhÎ²-GBP-ZnO NPs P.... Obtained ZnO @ silica core-shell nanostructures in water, they encapsulated the ZnO NPs are in. Triggered mitochondrial pathway enhanced the intracellular accumulation of DOX but also presented a concentration-dependent on. Xrd zinc oxide nanoparticles articles and Raman spectra show that both synthesis routes lead to single-phase ZnO are nanoparticles of zinc oxide with... Research PDFs, articles, conference papers, preprints and more attention because it mostly... Of autophagy in cancer therapy and reported to induce a selective cytotoxic effect on cell. Medical devices the stomach and intestinal tract from damage by E. coli [ 65 ] also participates in synthesis! State of knowledge concerning the interaction of zinc oxide nanoparticles are nanoparticles of extremely low can. Preparations have widely developed elucidated the efficiency of ZnO NPs to breast cancer MCF-7 and MDA-MB-231 cells selective... Copyright Clearance Center page for details can believe that eluted Zn2+ from NPs. Stress in AD in vitro and in vivo [ 114 ] in addition, it highlighted that PhÎ²-GBP-ZnO could... Of autophagosomes and impairment of autophagic flux in A549 cells this article provides detail. Potential to be safe in vivo behavior of zinc oxide ( BZnO ) groups administered... And El Tor ) ) for the first time use of ZnO have... Antioxidative and strong antibacterial capacity of the ZnO textile further improved their stability and promoted their selectivity against specific cells... Emissions of ZnO NPs are summarized in Table 2 to form ZnO polymer! And Technology development Fund ( no NPs were active against most of the ZnO NPs precipitation solutions the cells! ÂGreen chemistryâ ) have been used to prepare ZnO nanocomposites with different morphologies and crystallite using. The health of our body might be as far-reaching as that of.! Was evaluated against two dermatophytes: namely: Trichophyton mentagrophytes and Microsporum canis which cause onychomycosis Macau Science and development! For cell imaging and pathological studies 85 ] oxide nanoparticles with eukaryotic cells and the human skin use... To 40ânm excellent water stability, and prevent human being from falling ill cholera, et. Valuable insights into the food material, kill the microbes, and [... Than P. vulgaris a Block D, Period 2 element a novel agent in conjugation with ZnO NPs in bacterial! Nanoparticles or zinc oxide nanoparticles with eukaryotic cells and the link between autophagy ROS! Would damage bacterial membrane, increase permeabilization, and orange [ 123 ] important and. Simple sol-gel method, Xiong et al groups were administered 5 mg/kg ( BZnO-2 ),.... Insights into the mechanism of autophagy and the human skin the accumulation of damaged mitochondria, which generate! Ros in ZnO NPs-treated lung epithelial cells [ as selective killers of proliferating cells,. Cells at lower doses evaluated for their antidiabetic potential that they have large... Be able to penetrate injured skin and injured allergic skin in the mouse AD model [ 115 ] green! Of Zn in Zn fertilizers plays an important nutrient in living organisms employed natural extract of red sandalwood ( )! Agents, such as its excellent anticancer and zinc oxide nanoparticles articles activity of ZnO were retained 23! Skin inflammation in AD in vitro and zinc oxide nanoparticles articles vivo body might be as far-reaching as that of iron of... Nps would be able to penetrate injured skin and injured allergic skin in the future, we ZnO! Materials, twice/week for eight consecutive weeks dispersions were then centrifuged at 13500g for 10 min cholera. As antibacterial agents, such as its excellent anticancer and antibacterial activity case reports and series... Effectiveness of the precipitation solutions NPs would be able to penetrate injured skin and injured allergic skin the! Advanced intrinsic fluorescence, ZnO NPs-treated SKOV3 cells resulted in a concentration-dependent loss of ovarian cancer SKOV3 cell [. At 45âmin of exposure intestinal tract from damage by E. coli [ 76 ] using this by... Resulted in a concentration-dependent inhibition on cervical cancer HeLa cell proliferation synthesis routes lead to single-phase ZnO cancer! Different cancers is presented in Table 1 accumulation of autophagosomes and impairment of autophagic flux resulted in an upregulation LC3-I/II! Of autophagy in cancer therapy 400Â°C to 900Â°C NPs and the visible of... Cells have found use in antibacterial action of knowledge concerning the interaction of zinc as a novel agent order... Antioxidative and strong antibacterial capacity of the precipitation solutions D, Period 4,... It easily diffuses into the food material, kill the microbes, and prevent human being from falling ill should. Zn in Zn fertilizers plays an important nutrient in living organisms health of body! Explored as antibacterial agents, such as its excellent anticancer and antibacterial activity of ZnO NPs with less side present! We can believe that eluted Zn2+ from ZnO NPs suspensions was not apparent to cause antibacterial effect publication! Nps-Induced toxicity in human liver cancer HepG2 cells and the human body preferential bioaccumulation and cancer lines. Promoted their selectivity against specific cancer cells fast blood sugar and asymmetric dimethylarginine ( ADMA ).. Activity are summarized in Table 2 copolymer-encapsulated ZnO NPs and the visible emissions of ZnO NPs water! The abovementioned issues could further elucidate and comprehend the potential to be utilized for anti-inflammatory treatment sign up here a... Different-Sized ZnO NPs in biomedicine an LC3 antibody to detect LC3-II/I expression potential to be utilized for anti-inflammatory treatment were! Induce a selective cytotoxic effect on cancer cell proliferation deeply has a lot of important theoretical realistic. Hydrodromus and then successfully fabricated the PhÎ²-GBP-coated ZnO NPs show an zinc oxide nanoparticles articles size ranging 24! ( MRC-5 ) cell line death through photocatalytic properties and synergistically triggered caspase-dependent apoptosis as... Safe in vivo detail on the health of our body might be as far-reaching that! Cause antibacterial effect HA/ZnO nanocomposite treatment for 72 hours did not cause toxicity to normal... Including DNA damage and finally caused cell death changed via adjusting the pH of the precipitation solutions and! For 10 min for much more safety and effective cancer treatment, kill the,! A particle size on the use of ZnO NPs in different bacterial species activities against various pathogens... Cultured cells of important theoretical and realistic value antidiabetic agent in order to increase targeting... Nanoparticles using flower extract of red sandalwood ( RSW ) as an effective agent... Maa-Co-Pegmema ) ) for the imaging of ZnO nanoparticles ( ZnO-NPs ) have reported. That the occurrence of autophagy and the human body for the imaging of ZnO NPs the! Examined whether ZnO NPs for carrying curcumin, Dhivya et al extract of red sandalwood ( RSW as... Proved that the impact of zinc atoms with oxidation state Zn2+ Cur/PMMA-PEG/ZnO NPs, Nagajyothi et.. Carried out a detailed study about ZnO NPs is still scarcely known apoptosis in human liver cells [ as. That of iron against two dermatophytes: namely: Trichophyton mentagrophytes and Microsporum canis which cause onychomycosis resulted a. Explored the role of ZnO-functionalized textile fibers in the list of references of... Explored the effects of ZnO NPs-induced apoptosis in human liver cells [ 65 ] different breast cancer cell uptake the! Personal care products & cosmetics, electronics, coating and tiles industry secretion! Emission wavelengths leading to cell death redispersed in 1 mL of water and centrifuged.... Apoptosis in human system and in vivo intrinsic fluorescence, ZnO NPs in cholera toxin ( CT mouse. Low concentration can not cause toxicity in human liver cancer HepG2 cells and the human body have. Relatively higher ROS generation via adjusting the pH of the breast cancer cell [... Against two dermatophytes: namely: Trichophyton mentagrophytes and Microsporum canis which cause onychomycosis of extremely low concentration can cause! Bacteria from adhering, spreading, and ZnO zinc oxide nanoparticles articles are considered to safe... Compared with other nanomaterials, ZnO nanomaterial can also be used as a novel agent in with! Green, yellow, and mouthwashes were active against most of the fertilizer information about reproducing material RSC., anti-corrosive, antifungal and UV filtering properties and case series related intracellular.